Fig. 4From: Neutrophils are a main source of circulating suPAR predicting outcome in critical illnessImpact of inflammatory mediators on uPAR expression of human neutrophils and monocytes ex vivo. Primary human neutrophils (a, b) and monocytes (c) were isolated from healthy controls (n = 8), and uPAR expression was analyzed by flow cytometry. d Human neutrophils were incubated for 8 h either with 10% serum from healthy volunteers (control), interleukin-8 (IL 8, 100 ng/ml, in addition to 10% healthy human serum), macrophage supernatant (MP), or 10% sepsis serum (pooled from n = 4 sepsis patients. b Release of suPAR was analyzed in the supernatant of the neutrophil cultures. c Human macrophages were cultured for 5 days in 10% serum from healthy controls and subsequently exposed to 10% serum from sepsis patients for 48 h. As a positive control, macrophages were cultured for 6 days, followed by stimulation with LPS (100 ng/ml) + 10% healthy human serum for 24 h. d Release of suPAR in the supernatant from macrophage cultures with the different conditionsBack to article page