Fig. 6

The effect of dexamethasone or rapamycin on phosphorylation of Akt or c-Jun. a A549 cells were pretreated with dexamethasone (D) or rapamycin (R) at the indicated concentration for 1 h and then stimulated with CM. Total cell lysate was collected 24 h (upper panel) or 48 h (lower panel) after CM stimulation and then subjected to western blotting using the indicated antibody. The effect of combination of dexamethasone (10 nM) and rapamycin (1 nM) surpassed that of 10 times concentration of each drug in terms of the inhibition of iNOS, cox2, cleaved caspase 7, and phosphorylated H2AX. In addition, both of augmentation of phosphorylated Akt (p-Akt) and inhibition of phosphorylated c-Jun (p-c-Jun) were remarkable in combined treatment of dexamethasone and rapamycin. b A549 cells were pretreated with dexamethasone (10 nM, D), rapamycin (1 nM, R) or both (10 nM D/1 nM R) for 1 h and then stimulated with BSA (vehicle control) or CM. Total cell lysate was collected 24 h after stimulation and then subjected to western blotting using the indicated antibody