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Fig. 4 | Journal of Intensive Care

Fig. 4

From: Circulating histone H3 levels are increased in septic mice in a neutrophil-dependent manner: preclinical evaluation of a novel sandwich ELISA for histone H3

Fig. 4

Circulating histone H3 levels are mainly derived from neutrophils in CLP mice. a Neutrophil depletion was conducted by intravenous injection of 100 μg/mouse of anti-Ly-6G antibody at 72 and 24 h prior to blood cell counts. RBC, red blood cell (× 103 cells/μL); PLT, platelet (× 102 cells/μL); WBC, white blood cell (cells/μL). Differences in white blood cell (WBC) and neutrophil counts between anti-Ly-6G antibody-injected mice (neutrophil depletion, n = 8, mean ± SD) and isotype control antibody-injected mice (control, n = 5, mean ± SD) were analyzed by Welch’s t test. **p < 0.01. b Neutrophil depletion was achieved by intravenous injection of 100 μg/mouse of anti-Ly-6G antibody at 72 and 24 h prior to CLP. For assessment of cellular damage, the serum LDH levels at 24 h after CLP were examined. The serum LDH levels at 24 h after CLP did not differ significantly between neutrophil-depleted mice (n = 6, mean ± SD) and control mice (n = 10, mean ± SD). c Serum histone H3 levels at 24 h after CLP were examined in neutrophil-depleted mice (n = 8) and control mice (n = 10). Representative data of two independent experiments are shown. Differences in circulating histone H3 levels were analyzed by the Mann–Whitney U test. **p < 0.01

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