Skip to main content

Advertisement

Table 3 mRNA expression after TNF stimulation in human pulmonary artery endothelial cells

From: Alert cell strategy in SIRS-induced vasculitis: sepsis and endothelial cells

  0 min 15 min 30 min 1 h 4 h 8 h
ATG5 1 0.3 0.3 1.2 0.1 0.2
ATG8 1 4.1 4.5 4.0 5.5 0.6
ATG12 1 6.5 4.9 4.3 6.8 2.2
c-FOS 1 10.3 18.6 6.2 3.1 2.8
c-Jun 1 3.2 8.7 4.4 2.1 0.8
ERK1 1 1.6 1.1 0.6 0.5 0.6
FADD 1 1.8 3.2 3.6 2.8 1.2
ILAK1 1 7.3 12.6 10.3 10.1 2.9
ILAK2 1 2.1 1.8 1.6 1.2 0.5
ILAK4 1 2.2 1.8 1.6 1.4 0.6
MAPK14 1 3.8 3.6 3.1 3.5 1.4
MyD88 1 2.1 1.5 1.3 1.1 0.2
p62 1 5.8 6.8 7.2 6.8 2.1
p65 1 1.1 0.8 0.6 0.4 0.2
RIPK 1 3.3 2.2 1.8 2.4 0.8
STAT3 1 6.3 2.4 2.8 3.3 0.5
TLR2 1 5.8 3.6 5.3 3.6 1.2
TLR4 1 0.7 0.8 0.5 0.6 0.2
TAK1 1 3.8 4.3 3.7 3.6 1.2
TNFRSF1A 1 1.9 2.2 1.9 2.1 1.4
ULK1 1 1.5 1.2 1.3 1.8 0.9
  1. Human pulmonary artery endothelial cells (HPAEC) were observed in the time course in a separate medium after TNFα 100 ng/mL administration without steroid and the other anti-inflammatory molecules. At 8 h after TNFα stimulation, the endothelial cells were observed with shape change and cell death in approximately 35 %. High expression of mRNA of ATG8, ATG12, ILAK1, MAPK14, p62, TLR2, and TAK1 were observed in HPAEC through the time course after TNFα by reverse transcription polymerase chain reaction of the inner control of actin mRNA