Figure 5
From: Neutrophil cell death in response to infection and its relation to coagulation
Immunofluorescent staining of NETs. The bright-field (left upper panel) view of NETosis induced by 10 nM of PMA. Immunofluorescent staining by propidium iodine visualizes DNA ejected from the neutrophils (left bottom panel, same field as above). Phase-contrast view of the neutrophils stimulated by PMA (right upper panel) and the overlay image (right bottom panel) showing DNA distribution (red). Arrows indicate the expelled DNA. All scale bars represent 20-μm length.