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Fig. 1 | Journal of Intensive Care

Fig. 1

From: Serial change of neutrophil extracellular traps in tracheal aspirate of patients with acute respiratory distress syndrome: report of three cases

Fig. 1

Time course images of immunostaining of tracheal aspirates and changes of PaO2/FiO2 ratio, white blood cell count (WBC), and C-reactive protein (CRP) in case 1. Methylprednisolone (150 mg/day) was given intravenously from hospital days 8 to 18. Triple staining was performed with 4′,6-diamidino-2-phenylindole (DAPI), anti-human histone H3 mouse monoclonal antibody, and anti-human Cit-H3 rabbit polyclonal antibody. Blue: DAPI; red: histone H3; green: citrullinated histone H3. White arrowheads indicate NETs. Scale bar = 30 μm

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