Fig. 3

Circulating histone H3 levels are mainly derived from leukocytes in CLP mice. a Leukocyte depletion was achieved by intraperitoneal injection of 150 mg/kg and 100 mg/kg cyclophosphamide at 72 h and 24 h prior to blood cell counts, respectively. RBC, red blood cell (× 10³ cells/μL); PLT, platelet (× 10² cells/μL); WBC, white blood cell (cells/μL). Differences in WBC counts between cyclophosphamide-injected mice (leukocyte depletion, n = 5, mean ± SD) and saline-injected mice (control, n = 6, mean ± SD) were analyzed by Welch’s t-test. **p < 0.01. b Leukocyte depletion was conducted by intraperitoneal injection of 150 mg/kg and 100 mg/kg cyclophosphamide at 72 and 24 h prior to CLP, respectively. For assessment of cellular damage, the activities of AST, ALT, and LDH at 24 h after CLP or sham operation were examined. The AST, ALT, and LDH levels at 24 h after CLP did not differ significantly between leukocyte-depleted mice (n = 9, mean ± SD) and control mice (n = 10, mean ± SD). c Serum histone H3 levels at 24 h after CLP were examined in leukocyte-depleted mice (n = 9) and control mice (n = 10). Representative data of two independent experiments are shown. Differences in circulating histone H3 levels were analyzed by the Mann–Whitney U test. **p < 0.01