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Fig. 1 | Journal of Intensive Care

Fig. 1

From: Circulating histone H3 levels are increased in septic mice in a neutrophil-dependent manner: preclinical evaluation of a novel sandwich ELISA for histone H3

Fig. 1

Validation of the newly developed ELISA for detection of circulating histone H3 levels. a Intra-assay and inter-assay precisions were analyzed using serum samples containing histone H3 at concentrations of 10 and 100 ng/mL. The intra-assay (n = 20) and inter-assay (n = 10) CVs were 2.0–4.1% and 4.8–9.1%, respectively. OD, optical density; CV, coefficient of variation. b The detection limit determined by the mean ± 2.6 SD method was 2 ng/mL. c Linearity was observed in the range up to 250 ng/mL

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