Fig. 4
LA-1 inhibits migration of neutrophils on endothelial monolayers. HUVEC monolayers were grown on delta T dishes coated with 40 μg/mL type I collagen and TNF-α activated. Neutrophils were either TNF-α-activated and given no treatment, 15 μM LA-1, or DMSO vehicle control. Cell interactions were allowed to adhere at 37 °C and neutrophil migration dynamics were imaged. × 20 bright-field images were captured every 30 s for 20 min. Neutrophil migration paths were tracked in ImageJ and analyzed using ibidi’s Chemotaxis and Migration Tool (a). LA-1 treatment reduced neutrophil path length (b), displacement (c), and speed (d) regardless of donor. Two-way ANOVA with post hoc Tukey analysis, *p < 0.05 LA-1 vs. untreated or DMSO-treated neutrophils