Fig. 1
Endothelial barrier disruption by neutrophils isolated from healthy (n = 14), trauma (n = 8), and septic donors (n = 6) and protection by LA-1. Change in normalized resistance with respect to time. T = 0 represents time of neutrophil addition to HUVECs activated by TNF for 4 h. a Representative tracing of electrical resistance across activated HUVEC monolayers in the presence of neutrophils obtained from healthy, trauma, or septic donors. b Values are mean ± SEM of normalized resistance at 60 min following PMN addition. Analyses were performed evaluating the effect of neutrophils obtained from critically ill trauma patients (n = 8), from critically ill septic patients (n = 6), and from healthy controls (n = 14) and assayed in duplicate on each experimental day. c Representative ECIS tracing of neutrophils added to HUVEC monolayer 30 min after fMLP (10−6 M). d Values are mean ± SEM of normalized resistance at 60 min following PMN addition ± fMLP. e–g Representative tracings of neutrophils ± fMLP activation in the presence or absence of LA-1. e Neutrophils obtained from healthy donors. f Neutrophils obtained from trauma patients. g Neutrophils obtained from septic patients. h Values are mean ± SEM of normalized resistance at 60 min following PMN addition ± fMLP. *p < 0.05 vs. healthy unstimulated; **p < 0.05 vs. healthy fMLP-stimulated; #p < 0.05 vs. trauma unstimulated; ##p < 0.05 vs. trauma fMLP-stimulated; @p < 0.05 vs. septic unstimulated; and @@p < 0.05 vs. septic fMLP-stimulated. Statistical significance determined (p < 0.05) using three-way ANOVA with post hoc Tukey analysis