Fig. 3
From: Glycocalyx and its involvement in clinical pathophysiologies
Typical experimental methods used to analyze GCX/ESL function. a Fluorescent-labeled leukocytes in microvasculature. To quantify the leukocyte-endothelium interaction, fluorescence-labeled leukocytes in flowing blood were observed within a region of interest (ROI) during a 30-s video recording, and adhesive and/or rolling leukocytes were counted. b Permeable analysis using FITC dextran. To analyze vascular permeability, fluorescence-labeled dextran was injected and time-dependent changes in brightness within an ROI (yellow box) set over the interstitium were identified using image analysis software. (These images were originally obtained by H. Kataoka)