Table 1 The sequences of the PCR primers used in this study
From: Sepsis induces incomplete M2 phenotype polarization in peritoneal exudate cells in mice
Target gene | Sequence | |
|---|---|---|
TNF-α | Forward | CTGTAGCCCACGTCGTAGC |
Reverse | TTGAGATCCATGCCGTTG | |
IL-1β | Forward | TGTAATGAAAGACGGCACACC |
Reverse | TCTTCTTTGGGTATTGCTTGG | |
IL-6 | Forward | GAGGATACCACTCCCAACAGACC |
Reverse | AAGTGCATCATCGTTGTTCATACA | |
IL-12 p35 | Forward | CACCCTTGCCCTCCTAAACC |
Reverse | CACCTGGCAGGTCCAGAGA | |
IL-10 | Forward | TGAGGCGCTGTCATCGATTTCTCCC |
Reverse | ACCTGCTCCACTGCCTTGCT | |
IL-1ra | Forward | TCAGATCTGCACTCAATGCC |
Reverse | CTGGTGTTTGACCTGGGAGT | |
iNOS | Forward | ACATCGACCCGTCCACAGTAT |
Reverse | CAGAGGGGTAGGCTTGTCTC | |
ARG 1 | Forward | ATGGAAGAGACCTTCAGCTAC |
Reverse | GCTGTCTTCCCAAGAGTTGGG | |
MCP1/CCL2 | Forward | ACTGAAGCCAGCTCTCTCTTCCTC |
Reverse | TTCCTTCTTGGGGTCAGCACAGAC | |
CCL22 | Forward | GTGGCTCTCGTCCTTCTTGC |
Reverse | GGACAGTTTATGGAGTAGCTT | |
CCL24 | Forward | TGTGACCATCCCCTCATCTTGC |
Reverse | AAACCTCGGTGCTATTGCCACG | |
CCL17 | Forward | TAAGACCTCAGTGGAGTGTTC |
Reverse | AAATGCCTCAGCGGGAAG | |
GAPDH | Forward | TGCACCACCAACTGCTTAG |
Reverse | GGATGCAGGGATGATGTTC | |
SOCS1 | Forward | GTTGTAGCAGCTTGTGTCTG |
Reverse | TGGTTTGTGCAAAGATACTG | |
SOCS3 | Forward | TACTGAGCCGACCTCTCTC |
Reverse | AGCTGGGTCACTTTCTCATA |