ROCK-1 and HMGB-1. (A) Rho-associated kinase (ROCK)-1 mRNA, (B) ROCK-1 protein expression, (C) high-mobility group box 1 (HMGB-1) mRNA, (D) HMGB-1 protein expression. For protein expression, the average intensity of LV/SAL was taken as 100%. Each sample intensity as a percentage of the average was calculated (relative intensity). *P < 0.05 versus the respective LV group. n = number of rats. See Figure 2 for abbreviations.