Figure 6

Detection of polystyrene beads in glomeruli of kidneys and urine. Mice were randomized to dyed polystyrene bead injection (10⁸/kg, 6-μm dia.) (Dyed Bead) or dyed polystyrene bead injection (10⁸/kg, 6-μm dia.) 30 min after LPS injection (5 mg/kg) (L + DB) and treated as described. (A) Three, six, or twelve hours after injection, the kidney sections were obtained. Dyed Bead (n = 3), L + DB (n = 5). Picture shows one of the Sections 6 h after injection in L + DB. Dyed polystyrene beads are found in the glomeruli (arrows). Black bar, 100 μm. (B) In the sections obtained from Figure 6A, dyed polystyrene beads in the glomeruli were counted and presented per number of glomeruli. Dyed Bead (n = 3), L + DB (n = 5). (C) Mice were randomized to Dyed Bead or L + DB and treated as described. Urine 0–3, 3–6, and 6–12 h after injection was collected, and dyed polystyrene beads were counted and presented per weight of animals. Dyed Bead (n = 5), L + DB (n = 5). Asterisk, significantly different between Dyed Bead and L + DB at P < 0.0001. Number sign, significantly different from 3–6 h after injection of L + DB at P < 0.001.