Figure 2
From: IV injection of polystyrene beads for mouse model of sepsis causes severe glomerular injury
Histology. Mice were randomized to polystyrene bead injection (108/kg, 6-μm dia.) (Bead), LPS injection (5 mg/kg) (LPS), polystyrene bead injection (108/kg, 6-μm dia.) 30 min after LPS injection (5 mg/kg) (L + B), or saline injection (5 ml/kg) (Control) and treated as described. Forty-eight hours after injection, the liver, kidney, and spleen were removed. Sections of the liver and spleen were processed for hematoxylin and eosin (HE) staining. Sections of each kidney tissue were processed for HE staining and periodic acid methenamine silver (PAM) staining. Horizontal line is HE staining of liver (low and high magnification), HE stain of kidney, PAM staining of kidney, and HE staining of spleen (low and high magnification), respectively. Vertical lines are Control, Bead, LPS, and L + B, respectively. Arrows indicate neutrophils. Control (n = 4), Bead (n = 4), LPS (n = 4), L + B (n = 5). LM, low magnification; HM, high magnification; Black bar, 100 μm.