Figure 3
From: Neutrophil cell death in response to infection and its relation to coagulation
Immunofluorescent staining of necrotic neutrophils. Bright-field (left panel) and overlay (middle panel) images showing DNA distribution in mouse neutrophils stimulated by lipopolysaccharide. In the necrotic neutrophils (white arrows), DNA spreads in the cytoplasm and are stained by ID-red (red), while other cells keep the granules and DNA resides in nuclei (black arrows). The right panel shows the combined image of the selected cells. Microscopic pictures were taken 3 h after stimulation. All scale bars represent 20-μm length.